New biosensors for detection of bacteria
Student: Rudy Acosta
Degree: M.S., December 2020
Major Professor: Dr. Z. Ryan Tian
Physical & Chemical Sensors
Biological Materials & Processes
- Current methods in the market for bacteria detection are time consuming, expensive, user unfriendly, and tend to produce false negative/positive results more often than desired.
- Compact and inexpensive bacterial sensors have been long-overdue to industry, homeland security, and healthcare sectors.
By modifying the radio frequency identification (RFID) tags, volatile organic compounds (VOC) of different bacteria can be identified by monitoring the RFID signals in real-time.
- Deposit rGO-TiO2/polymer nanocomposite thin films (sensing layer) on the surface of an RFID tag’s hot spot.
- Using the modified tag to detect the bacteria-generated VOCs
- In parallel, integrating the tag with a custom-made flow cell for detecting bacteria e.g. salmonella, E. coli, bacillus B (see below)
- As shown below, there could be a linear correlation between the tag signal’s intensity and the target analyte’s descending concentration.
- Our new sensory nanomaterial made the tag to show different signals for each of the VOCs at various concentrations.
Conclusions and Future Work
When interacting with the RFID sensor, bacteria-generated VOCs each shows a characteristic impedance and frequency.
Further testing will be conducted to find the limit of detection (LOD) of bacterial cells in a new microfluidic device.